Journal: JAMA Pediatrics

Article Title: Longitudinal Changes in Epigenetic Age Acceleration Across Childhood and Adolescence

doi: 10.1001/jamapediatrics.2024.3669

Figure Lengend Snippet: List of Covariates

Article Snippet: Saliva samples were assayed for genome-wide DNA methylation profiling using Illumina EPIC or 450K methylation arrays for 874 and 1165 participants, respectively.

Techniques: Control, DNA Methylation Assay, Produced, Transformation Assay

Journal: Nature Medicine

Article Title: A prognostic neural epigenetic signature in high-grade glioma

doi: 10.1038/s41591-024-02969-w

Figure Lengend Snippet: a , Schematic of the study workflow. In humans ( n = 5,047) diagnosed with a CNS tumor we performed deconvolution using DNA methylation arrays (850k or 450k) for determining the neural signature. IDH-wild-type glioblastomas were stratified into subgroups with a low- or high-neural signature for further analyses. b , Epigenetic neural signature in all CNS tumor entities ( n = 5,047). c , Dichotomization of the combined dataset from Capper et al. and three institutional cohorts (Hamburg, Berlin and Frankfurt, all Germany) into low- and high-neural glioblastomas. The black line indicates a median neural score of all included patients with glioblastoma ( n = 1,058) and represents the cutoff (0.41) for stratification into low- and high-neural glioblastoma. d, External validation of the cutoff value using the TCGA-GBM dataset ( n = 187). The black line indicates the median neural score. e – i , Survival analysis of patients with low- and high-neural glioblastoma treated by radiochemotherapy after surgery. e , Overall survival (OS) of 363 patients with glioblastoma of the internal clinical cohort. log-rank test, P = 0.000005. Error bands represent 95% CI. f , PFS of 226 patients with glioblastoma of the internal clinical cohort. log-rank test, P = 0.0233. Error bands represent 95% CI. g , Overall survival of 187 patients with glioblastoma of the TCGA-GBM cohort. log-rank test, P = 0.0017. Error bands represent 95% CI. h , i , Forest plots illustrating multivariate analysis of patients with glioblastoma from the internal clinical cohort. Means are shown by closed circles and whiskers represent 95% CI. GTR, gross total resection; PR, partial resection; MGMT, O 6 -methylguanine-DNA-methyltransferase.

Article Snippet: Forty-four treatment-naive patients with glioblastoma (mean age 65 ± 9 years) underwent rs-fMRI before surgery, with tumor tissues subsequently analyzed for genome-wide DNA methylation patterns using the Illumina EPIC (850k) array.

Techniques: DNA Methylation Assay, Biomarker Discovery